Can you transfect siRNA?
Gene silencing or knockdown can be done easily now in most cell types, but there are a few things you need to consider before starting: First, you’ll need to design the siRNA (small interferring RNA) against your gene.
How do you dissolve siRNA?
General considerations: Dissolve siRNA in sterile water and prepare 5 µl aliquots, store at –80°C. For use, dilute siRNA in siRNA dilution buffer (diluted siRNA is not stable, use only once). 5x siRNA buffer aliquots (Dharmacon) are stored at 4°C in the cell culture lab.
How do you mix siRNA?
To dilute the 5x siRNA Buffer to 1x siRNA Buffer, mix four volumes of sterile RNase-free water with one volume of 5x siRNA Buffer. The composition of the 1x siRNA Buffer is 60 mM KCl, 6 mM HEPES-pH 7.5, and 0.2 mM MgCl2.
How do you create a siRNA experiment?
Top 4 ways to make your siRNA experiment a success
- Choose appropriate siRNA reagents and delivery methods that are adapted to your cell type.
- Use appropriate positive and negative controls in each and every experiment.
- Optimize siRNA delivery conditions.
- Confirm your knockdown on the mRNA level using qPCR.
How long can you store siRNA?
siRNA may be stored for up to 6 months at -20 °C. In a non-frost-free freezer, we recommend that the solution is not freeze or thawed more than 3–5 times. In the lyophilized form, the duplexes are stable for much longer at -20 °C.
How effective is siRNA transfection reagent?
siRNA Transfection Reagent is rated 4.8 out of 5 by 19 . Effective in both serum-containing medium and serum-free medium, offering high efficiencies with minimal cytotoxicity
Is the siRNA transfection reagent sc-29528 appropriate for Caco-2 cell line?
Thank you for your question. siRNA Transfection Reagent: sc-29528 is suitable for a wide variety of mammalian cell lines. It works best with adherent cell lines such as PC-3 and should work well when transfecting these cells. Is the siRNA transfection reagent sc-29528 appropriate for Caco-2 cell line?
What is a transfection reagent?
In some cases, transfection reagents can include molecules that are designed to help desired compounds enter a cell. For example, a lipofection transfection reagent will include chains of liposomes that encapsulate a given nucleic acid sequence and allow it to be endocytosed by a cell.
How to do gene silencing or knockdown with siRNA?
siRNA Transfection. Gene silencing or knockdown can be done easily now in most cell types, but there are a few things you need to consider before starting: First, you’ll need to design the siRNA (small interferring RNA) against your gene. Second, you’ll need to deliver it to the cells effectively with a siRNA transfection procedure.