How do you calculate how much stock solution you need?
To determine the volume of stock solution needed, divide the number of moles of glucose by the molarity of the stock solution.
How do you calculate the dilution of a solution?
The formula for calculating a dilution is (C1) (V1) = (C2) (V2) where…
- C1 is the concentration of the starting solution.
- V1 is the volume of the starting solution.
- C2 is the concentration of the final solution.
- V2 is the volume of the final solution.
How do you prepare a stock solution?
A stock solution is prepared by weighing out an appropriate portion of a pure solid or by measuring out an appropriate volume of a pure liquid, placing it in a suitable flask, and diluting to a known volume. Exactly how one measure’s the reagent depends on the desired concentration unit.
How much stock solution and diluent are needed?
Multiply the final desired volume by the dilution factor to determine the needed volume of the stock solution. In our example, 30 mL x 1 ÷ 20 = 1.5 mL of stock solution. Subtract this figure from the final desired volume to calculate the volume of diluent required–for example, 30 mL – 1.5 mL = 28.5 mL.
How do I make a 10 mg/mL stock solution?
To prepare a concentration of 10 µg/ml, pipette out 10 µl of the drug in a test tube using a micropipette. Then dilute it with 990 µl (making a total volume of 1 ml) of the solvent (ethanol, methanol, water etc) you are going to use in your experiment. Similarly, do for 20 µg/ml.
How do I make a 10 mg/ml stock solution?
How do you make a 4% solution?
4% NaOH →Mean 4 grams of NaOH is dissolved in 100 ml of the solution. 4g ——> 100 ml? ——–> 50 ml The Weight in grams of NaOH needed to prepare 4% NaOH is = (4 x 50)/100 = 2 g. So, 2 grams of NaOH is dissolved in little water and the volume made up to 50 ml.
How do you prepare stock solution in ppm?
You may prepare the 1ppm stock solution first by dissolving 1 mg of solute into 1000 mL of the solvent. After that, you can prepare the solution of any desired concentration using the formula, M1V1=M2V2. I hope this answer will help you.