What is a leaf explant?

An explant is a part of the plant by which a whole plant can be produced through plant tissue culture technique. From: Chickpea: Crop Wild Relatives for Enhancing Genetic Gains, 2020.

What are explants give example?

In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. The term explant can be applied to samples obtained from any part of the organism.

What is a callus in plants?

callus, In botany, soft tissue that forms over a wounded or cut plant surface, leading to healing. A callus arises from cells of the cambium. When a callus forms, some of its cells may organize into growing points, some of which in turn give rise to roots while others produce stems and leaves.

How do you choose explants?

Types of Explants used in Tissue Culture

  1. Age of the Organ used to Source the Explant. The age of the explant is an important factor when choosing the right explant.
  2. When the Explant was Collected.
  3. Size and Place of the Explant.
  4. Quality of the Source Plant.
  5. Plant Genotype.

What are Soma clones?

Somaclones are the plants that are produced through tissue culture and are genetically identical to the parent plant from which they are produced and cultured.

What is Cotyledonary leaf?

1. Botany A leaf of the embryo of a seed plant, which upon germination either remains in the seed or emerges, enlarges, and becomes green. Also called seed leaf.

What is Protoplasting?

Protoplast (from Ancient Greek πρωτόπλαστος (prōtóplastos) ‘first-formed’), is a biological term coined by Hanstein in 1880 to refer to the entire cell, excluding the cell wall. Protoplasts can be generated by stripping the cell wall from plant, bacterial, or fungal cells by mechanical, chemical or enzymatic means.

What are explants biology?

Explant is referred to as the cell or tissue which is taken from a particular body and then placed in a culture medium for growth. In terms of plants, the explant is the small pieces of plant part and issues that are aseptically cut and then they are kept in a nutrient medium.

What is leaf callus?

Plant callus (plural calluses or calli) is a growing mass of unorganized plant parenchyma cells. In living plants, callus cells are those cells that cover a plant wound.

What is callus and its usage?

Definition of callus (Entry 1 of 2) 1 : a thickening of or a hard thickened area on skin or bark. 2 : a mass of exudate and connective tissue that forms around a break in a bone and is converted into bone in healing. 3 : soft tissue that forms over a wounded or cut plant surface.

What types of explants are used in plant tissue culture?

There are various types of explants frequently used for regeneration purpose: nodal segments, apical meristems, roots, cotyledons, embryo, leaf disc, leaf blade, pedicle, petiole, anther, ovary etc.

What is micropropagation?

Micropropagation is the rapid vegetative propagation of plants under in vitro conditions of high light intensity, controlled temperature and a defined nutrient medium. The technique has been applied to a substantial number of commercial vegetatively propagated plant species.

What is the meaning of explant?

transitive verb. : to remove (living tissue) especially to a medium for tissue culture. explant. noun. ex·​plant | ˈek-ˌsplant . Definition of explant (Entry 2 of 2) : living tissue removed from an organism and placed in a medium for tissue culture.

How important is leaf area for photosynthetic ability of explants?

Photosynthetic ability of explants is critical, especially regarding the initial growth of plantlets. The enhanced net photosynthetic rates of explants could stimulate new root and axillary shoot development from the explants. Leaf area is, therefore, an important quality variable of explants.

How are explants made?

Explants are prepared by dissecting and mincing placental tissue into small masses, followed by plating into appropriate tissue culture wells and incubation for a finite period of time (Miller et al., 2005; Aplin, 2006).

What is explant culture?

Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding extracellular matrix to more accurately mimic the in vivo environment e.g. cartilage explant culture, or blastocyst implant culture. Historically, explant culture has been used in several areas of biological research.