Where are esterases found in the body?
Esterases, Including Butyrylcholinesterase (Pseudocholinesterase) Esterases catalyze hydrolysis of specific molecules containing the R1-CO-O-R2 ester group, as well as amides, hydrazides, and carbamates. In human physiology, esterases are distributed in the liver, erythrocytes, plasma, and the gastrointestinal tract.
What is the substrate used in the specific type cytochemical stain of esterase?
Abstract. Blood and bone-marrow smears from adult patients with acute leukemias were stained for esterase reaction, consecutively with naphthol AS D-chloracetate (chloracetate esterase) followed by alpha naphthyl butyrate (nonspecific esterase). The two substrates were, respectively, granulocyte- and monocyte-specific.
Where does esterase cleave?
Esterases cleave ester bonds in lipids and phosphatases cleave phosphate groups off molecules. An example of crucial esterase is acetylcholine esterase, which assists in transforming the neuron impulse into acetic acid after it the hydrolase breaks the acetylcholine into choline and acetic acid.
Why are cytochemical stains important?
cytochemical stains are the special stains that can be performed on peripheral blood smears and bone marrow smears. They play a very important role in diagnosis, differentiation and classification of Leukemias. 2) Immunocytochemical stains ie they identify cell specific antigens with the help of antibodies.
What is hydrolases function?
Hydrolases are the group of enzymes that catalyze bond cleavages by reaction with water. The natural function of most hydrolases is digestive to break down nutrients into smaller units for digestion.
What cytochemical stain is used to help differentiate a Leukemoid reaction from CML?
Leukocyte alkaline phosphatase (LAP) activity can be used to differentiate a leukemoid reaction from chronic myelogenous leukemia.