How long is propidium iodide good for?

Propidium Iodide (PI) Fluorescence Counterstain Protocol Store stock solution at 4 ºC (or aliquot and store at -20 ºC), protecting from light. When handled properly, solutions are stable for many years.

How do you store propidium iodide solution?

Store in refrigerator (2–8°C) and protect from light.

Why does propidium iodide stain dead cells?

Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population. PI binds to DNA by intercalating between the bases with little or no sequence preference.

How does flow cytometry measure cell viability?

Cell viability is assessed by flow cytometric measurement of cellular fluorescence after staining with fluorescein diacetate and propidium iodide in isotonic solution. The number of viable cells per ml of culture is determined by a timed count of viable cells and from knowledge of the flow cytometer sample flow rate.

How long do stained cells last?

Cells stained for surface antigens and then fixed with formaldehyde at 1 % (final concentration) should be stored for at least 24 hours (in the dark and at 4°C) and then read on a flow cytometer within 2 weeks (although most cells with most stains will probably last much longer).

Is propidium iodide a reliable indicator of cell viability?

and industry using approaches where propidium iodide (PI) serves as dead cell indica- tor. The reliability of PI uptake as a cell viability indicator for dead (PI permeable) and viable (PI impermeable) bacteria was tested using two soil bacteria, the gram2Sphingo- monas sp. LB126 and the gram1Mycobacterium frederiksbergense LB501T. Bacterial

What is propidium iodide (PI)?

Propidium iodide (PI) is a membrane impermeant dye that is generally excluded from viable cells. It binds to double stranded DNA by intercalating between base pairs.

What is propidium iodide flow cytometry?

Propidium Iodide Cell Viability Flow Cytometry Protocol Flow Cytometry Cell Viability Overview Flow cytometry is a quick and reliable method to quantify viable cells. Determining cell viability is an important step when evaluating a cells response to drug treatments or other environmental factors.

Is propidium iodide safe for FACs?

Note: Propidium iodide is a suspected carcinogen and should be handled with care. The dye must be disposed of safely and in accordance with applicable local regulations. Harvest cells and aliquot up to 1 x 10 6 cells/100 μL into FACS tubes.