What bacteria does Enterotube test for?
The Enterotube™ II is an example of a rapid, multi test system used in identification of unknown oxidase- negative, gram- negative, rod shaped bacteria of the family Enterobacteriaceae.
How do you read the Enterotube results?
The presence of acetoin, a positive result, is indicated by the development of a red color within 20 minutes, a negative reaction will appear colorless or light amber. The use of Barritt’s reagent in the VP chamber will be performed after all the results are read for the other chambers in the enterotube.
What is an Enterotube?
The Enterotube™ II is an example of a rapid, multi test system used in identification of unknown oxidase- negative, gram- negative, rod shaped bacteria of the family Enterobacteriaceae. It consists of a tube with a flat side and contains 12 compartments for different biochemical tests.
What Enterobacteriaceae are of medical significance?
Enterobacter species are responsible for causing many nosocomial infections, and less commonly community-acquired infections, including urinary tract infections (UTI), respiratory infections, soft tissue infections, osteomyelitis, and endocarditis, among many others.
What is the purpose of carbohydrate fermentation test?
The carbohydrate fermentation test is used to determine whether or not a bacteria can utilize a certain carbohydrate. It tests for the presence of acid and/or gas produced from the fermentation of a single particular carbohydrate.
Why do we use Enterotube?
It was concluded that the Enterotube system provides a simple, reliable, and rapid method for the presumptive identification of Enterobacteriaceae. The major advantage of the Enterotube is that all tests are done simultaneously by inoculation from a single isolated colony.
What type of specimen is used to inoculate an Enterotube?
Enterotubes: what do they test for? Used to identify Enterobacteriaceae from colonies o agar that have been on agar that have been inoculated from urine, blood, and sputum. The major advantage of the Enterotube is that all tests are done simultaneously by inoculation from a single isolated colony.
What causes Enterobacter infection?
The source of infection may be endogenous (via colonization of the skin, gastrointestinal tract, or urinary tract) or exogenous, resulting from the ubiquitous nature of Enterobacter species.
Does Enterobacter cause UTI?
What does a positive fermentation test mean?
Positive: After incubation the liquid in the tube turns yellow (indicated by the change in the color of the phenol red indicator). It indicates that there is drop in the pH because of the production of the acid by the fermentation of the carbohydrate (sugar) present in the media.
What is the principle of fermentation test?
The principle of carbohydrate fermentation states that the action of organism on a carbohydrate substrate results in acidification of the medium, detected by a pH indicator dye.