What is a confocal lens?

In optics, it means that one focus or image point of one lens is the same as one focus of the next lens.

How much does a confocal cost?

Prices may range anywhere from under $750 to over $89,000, depending on features. When considering confocal microscopes, there are more distinctive features associated with different models.

Why is it called confocal?

In contrast, a confocal microscope uses point illumination (see Point Spread Function) and a pinhole in an optically conjugate plane in front of the detector to eliminate out-of-focus signal – the name “confocal” stems from this configuration.

How does a confocal work?

Confocal microscopy uses light from a laser through the objective of a standard light microscope to excite a specimen within a narrow plane of focus. Any emission of light from out-of-focus planes is rejected by the pinhole, or confocal aperture. A simplified lightpath for a confocal microscope is illustrated below.

What are the disadvantages of confocal microscopy?

Disadvantages of confocal microscopy are limited primarily to the limited number of excitation wavelengths available with common lasers (referred to as laser lines), which occur over very narrow bands and are expensive to produce in the ultraviolet region.

How much is Leica confocal?

$79,999
Leica Microsystem’s high resolution confocal imaging system is available to you even if you are working with a small budget. For a limited time, get the Leica TCS SPE Personal Confocal at the starting price of $79,999, bringing your lab everything needed to get started with confocal imaging.

Are laser scanning confocal microscopes expensive?

“Confocal microscopes are very expensive and large, require more significant training to operate, and are generally slower to use.”

What is the disadvantage of confocal microscopy?

How does a laser confocal work?

Confocal microscopy uses light from a laser through the objective of a standard light microscope to excite a specimen within a narrow plane of focus. Any emission of light from out-of-focus planes is rejected by the pinhole, or confocal aperture.