What is a qPCR machine used for?

qPCR stands for quantitative polymerase chain reaction and is a technology used for measuring DNA using PCR.

What is Rotor Gene Q?

QIAGEN’s real-time PCR cycler, the Rotor-Gene Q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands. Together with optimized QIAGEN kits for real-time PCR, the Rotor-Gene Q enables streamlined analysis for a wide range of applications.

Are PCR machines expensive?

A simple PCR machine like Bio-Rad T100 thermal cycler has a list price of 4912 USD (with a promotional price of 2595 USD in the US) as of Jan 30, 2019. The cost of rtPCR systems ranges anywhere from 15,000$ for some RotorGene models to over 90,000$ for QuantStudio 12k.

How much does a PCR primer cost?

Service Details

Service Name Unit Unit Price
Primer Synthesis (25 nmol) 1 Base $0.53*
Primer Synthesis (100 nmol) 1 Base $1.05*
Primer Synthesis (250 nmol) 1 Base $1.88*
sgRNA PCR Primer Pair Design & Synthesis Service (25 nmol/primer) 1 Pair $148.50

How does a thermal cycler help with the process of PCR?

A thermocycler or PCR machine is a laboratory apparatus used for PCR. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then rises and lowers the temperature of the block in discrete, pre-programmed steps.

How RT PCR machine works?

The machine cycles through temperatures that heat and cool the mixture to trigger specific chemical reactions that create new, identical copies of the target sections of viral DNA. The cycle is repeated over and over to continue copying the target sections of viral DNA.

What are the types of PCR machine?

Types of PCR Machines

  1. Conventional PCR machine. The conventional polymerase chain reaction is used to amplify a target DNA sequence to several million in a short amount of time, usually in just 2-3 hours.
  2. qPCR.
  3. Reverse Transcription.
  4. Nested.
  5. Hot Start.
  6. Digital (dPCR)