What is annexin V used for?
Annexin A5 (or annexin V) is a cellular protein in the annexin group. In flow cytometry, annexin V is commonly used to detect apoptotic cells by its ability to bind to phosphatidylserine, a marker of apoptosis when it is on the outer leaflet of the plasma membrane.
What is Annexin V binding buffer?
The Annexin V Binding Buffer is a 10X concentrate composed of a 0.2 µm sterile filtered 0.1M Hepes (pH 7.4), 1.4M NaCl, and 25 mM CaCl2 solution. Prior to staining cells, an appropriate quantity of a 1X working solution should be made by diluting the 10X concentrate 1:10 with distilled water.
What is Pepe annexin V?
PE Annexin V – Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. Search Login/Register ▿ My Cart(0) Products Learn Support Quality About Us Contact Us Custom Solutions Custom Reagents Custom Services Custom Requests Form United States
Does the maxpar® labeling protocol work with human annexin V?
Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V. For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.
What is the function of annexin V?
Description Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner.
What type of pe do you use for annexin-phosphatidylserine binding?
Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C. What type of PE do you use in your conjugates? We use R-PE in our conjugates. Why do I need to use Annexin V Binding Buffer? Annexin V binding requires the presence of calcium in the solution.