What is LOX activity?

The LOX activity assay protocol uses a proprietary LOX substrate that releases hydrogen peroxide upon transformation by the LOX present in the sample. Hydrogen peroxide is in turn detected using a red fluorescence substrate for HRP-coupled reactions.

What is the biochemical reaction catalyzed by lysyl oxidase which is an enzyme essential for Desmosine crosslinking?

Lysyl oxidase catalyzes the oxidative deamination of specific lysyl residues in tropocollagen and tropoelastin.

What is LOX pathway?

The enzymes and receptors of the LOX pathway play a significant role in cell proliferation, differentiation and regulation of apoptosis through multiple signaling pathways and have been reported to be involved in various cancers, including prostate, colon, lung and pancreatic cancers.

How does collagenase break down collagen?

With their ability to cut the triple helical protein chains of collagen, collagenase enzymes work by targeting all forms of mammalian collagen, resulting in cell isolation. More specifically, collagenase enzymes work by degrading the collagen found in the extracellular matrix.

Do humans have collagenase?

Human skin fibroblasts secrete collagenase as two proenzyme forms (57 and 52 kDa). The minor (57-kDa) proenzyme form is the result of a partial posttranslational modification of the major (52-kDa) proenzyme through the addition of N-linked complex oligosaccharides.

What is lox cell?

LOX-IMVI cells are genetically characterized by lack of the Y chromosome and trisomy 7 (2), and are heterozygous for the BRAF V600E melanoma driver mutation (4). LOX-IMVI cells are amelanotic and express the human melanoma marker GD3 ganglioside, a factor in metastatic potential of malignant melanoma (5).

Why is lysyl oxidase important for the respiratory system?

“Lysyl oxidase is essential for normal development and function of the respiratory system and for the integrity of elastic and collagen fibers in various tissues”. The American Journal of Pathology. 167 (4): 927–36. doi: 10.1016/S0002-9440 (10)61183-2.

Does lysyl oxidase cause osteolathyrism?

Its inhibition can cause osteolathyrism, but, at the same time, its upregulation by tumor cells may promote metastasis of the existing tumor, causing it to become malignant and cancerous . In the yeast strain Pichia pastoris, lysyl oxidase constitutes a homodimeric structure.

What is the role of lysyl oxidase in human Collagene III promoter transcription?

Lysyl oxidase activates the transcription activity of human collagene III promoter. J. Biol. Chem. 2000;275:36341–36349. [PubMed] [Google Scholar] 115.

What is lysyl oxidase (LOX)?

From Wikipedia, the free encyclopedia Not to be confused with Lipoxygenase. Lysyl oxidase (LOX), also known as protein-lysine 6-oxidase, is an enzyme that, in humans, is encoded by the LOX gene. It catalyzes the conversion of lysine molecules into highly reactive aldehydes that form cross-links in extracellular matrix proteins.