What is the wavelength of FITC?
FITC emits fluorescence from 475 to 650 nm, peaking at 525 nm, which falls in the green spectrum.
What is the peak wavelength of FITC excitation light?
FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, giving it a green color.
What color does Cy3 fluorescence?
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC).
What is excitation wavelength fluorescence?
The wavelength of excitation monochromator is set to a wavelength of known absorption by the sample, and the wavelength of the emission monochromator is scanned across the desired emission range and the intensity of the fluorescence recorded on the detector as a function of emission wavelength.
What wavelength does GFP absorb and emit?
A mechanism for the fluorophore formation has been proposed (3) but needs to be confirmed by further studies. GFP absorbs blue light at 395 nm, with a smaller peak at 475 nm, and emits green light at 508 nm with a quantum yield of 0.72–0.85 (12, 13).
Is FITC a GFP?
Available Options. Green Fluorescent Protein (GFP) Antibody (FITC) is a Rabbit Polyclonal antibody conjugated to FITC against GFP.
What is the wavelength of a Cy3 laser?
… passes wavelengths between 535 and 575 nm (Fluores- cein) and 575-655 nm (Cy3). For the Cy5 the 633 nm wavelength line of a HeNe laser was used for excita- tion, while emission was measured through a filter, which passes wavelengths above 660 nm.
What is Cy3 dye?
The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.
What is the function of FITC in FRET?
It is used as an acceptor in fluorescence resonance energy transfer (FRET). Fluorescent dyes are the primary means of labeling biomolecules. The most common such dyes are FITC derivatives, which have a wide range of applications in ﬂuorescence microscopy, ﬂow cytometry, and immunoﬂuorescence-based assays.
How does FITC react with lysine?
After the selective unmasking of a protecting group, FITC might react with either lysine, an ornithine side chain, or with a primary amino group at the N-terminus of the growing peptide.