Which technique are used in downstream processing?

The purification of the product, the so-called downstream process (DSP), tends to be one of the most costly aspects of modern bioprocessing, especially in the case of proteins. In such cases, chromatography is still the major tool on all levels of the DSP from the first capture to the final polishing step.

What is chromatography in downstream processing?

In many biopharmaceutical companies, chromatographic purification in downstream processing is a key focus for optimization studies. The objective is to streamline the process, which may be achieved by the elimination of intermediate unit operations.

What is a basic technique for downstream?

Typical operations to achieve this are filtration, centrifugation, sedimentation, precipitation, flocculation, electro-precipitation, and gravity settling.

What is downstream processing PPT?

Downstream processing • The various stages of processing that occur after the completion of the fermentation or bioconversion stage, including separation, purification, and packaging of the product. 4.

Which of the following operations is a part of downstream processing?

Downstream processing is the recovery and purification of biochemical products with proper treatment. It is a series of events which includes cell separation, filtration, product recovery, extraction of product and purification and then treatment of product by chemical, physical and biological means.

What is filtration in downstream processing?

Filtration is a method of separating suspended particles from a liquid or gas using porous medium which retain the particles but allows the liquid or gas to pass through. The solid particles deposited on the filter form a layer, which is known as Filter cake.

What are the unit operations in downstream processing?

Downstream processing refers to the series of unit operations used to isolate, purify, and concentrate the product. Downstream processing often determines the economic feasibility of the process. The first operation is cell separation, which can be done by cross-flow microfiltration.

What is downstream processing class 12?

Downstream processing refers to the process of purification and separation of products of recombinant DNA technology. Suitable preservatives are added to the product . Proper quality check is maintained. In the case of drugs and medicines proper clinical trials are conducted.

What are the steps of downstream processing of an intracellular product?

Steps of downstream processing

  • Separation.
  • Cell disruption.
  • Extraction.
  • Isolation.
  • Purification.
  • Drying.

What are the chromatographic techniques?

Various chromatography methods have been developed to that end. Some of them include column chromatography, thin-layer chromatography (TLC), paper chromatography, gas chromatography, ion exchange chromatography, gel permeation chromatography, high-pressure liquid chromatography, and affinity chromatography [6].

What are the different types of chromatographic techniques?

Types of Chromatography

  • Adsorption Chromatography.
  • Thin Layer Chromatography.
  • Column Chromatography.
  • Partition chromatography.