Do CHO cells need serum?
The common CHO cell culture usually requires 10% fetal bovine serum, which can increase the risk of contamination and be unfavorable for protein purification during the downstream process (Kim et al., 2020). Hence, serum-free medium (SFM) for CHO culture is necessary for RTP production.
Why is serum-free media desirable in cell line protein production?
Wellens: It is indeed desirable to produce proteins in a serum-free environment. It will help reduce risks of virus contamination and it will ease the difficulties encountered in the downstream processing step.
What is serum containing media?
Serum-containing media are supplemented with serum, often from fetal calves, which contains growth factors and hormones that stimulate cell growth and function and help with cell attachment. The composition of serum varies, so testing is required to maintain the quality of each batch.
What is the role of serum in cell culture?
Serum is vitally important as a source of growth and adhesion factors, hormones, lipids, and minerals for the culture of cells in basal media. In addition, serum also regulates cell membrane permeability and serves as a carrier for lipids, enzymes, micronutrients, and trace elements into the cell.
Why is serum-free medium used?
Advantages of using serum-free media include a more consistent performance, increased growth and/or productivity, better control over physiological responsiveness, and to reduce risk of contamination by serum-borne adventitious agents in cell culture.
What is serum media?
Why is serum free medium used?
Why is serum added to cell culture media?
Why is serum-free media used for CHO cell culture?
The common CHO cell culture usually requires 10% fetal bovine serum, which can increase the risk of contamination and be unfavorable for protein purification during the downstream process (Kim et al., 2020). Hence, serum-free medium (SFM) for CHO culture is necessary for RTP production.
Which serum-free media for CHO-K1 cell growth?
In this study, seven commercially available serum-free media (EX-CELL, ISF-I, CD CHO, CDM4CHO, CHO-III-A, Octomed and HybridoMed) were evaluated and compared for cell … Comparison of commercial serum-free media for CHO-K1 cell growth and monoclonal antibody production Int J Pharm.
How can I increase recombinant protein yield in CHO cells?
In recent years, some special alternative additives have been used to increase recombinant protein yields in CHO cells. The more common special additives mainly originate from some small molecule chemical compounds and some effective components of hydrolysate (Kumar et al., 2007).
What is the growth rate of Cho dukxb11 cells?
The serum-free formulation supports excellent growth of CHO DUKXB11 cells at low (23cells/cm2) and high (2 x 10(4) cells/cm2) seeding densities characterized by a generation time of 10-12h, and, after addition of 0.2% pluronic F-68, the growth of a recombinant suspension cell line derived from DUKXB11.