How do microfluidic chambers work?
Basic microfluidic cell culture chamber with narrow microchannels. In this configuration, cells are injected/pumped using high pressure or manual syringe inside tight channels with sizes a little bit smaller than cells. Once cells are trapped, medium or drugs can be perfused at lower pressure through the microchannels.
What is a microfluidic chamber?
The microfluidic chamber consists of a molded elastomeric polymer placed on a glass coverslip (Fig. 1A). A physical barrier with embedded microgrooves connects the somal and axonal compartments. Microgrooves that connect the compartments allow the passage of neurites to the axonal compartment, but not of cell bodies.
What is the main mechanism through which the paper based microfluidic devices work?
Paper-based microfluidics are microfluidic devices that consist of a series of hydrophilic cellulose or nitrocellulose fibers that transport fluid from an inlet through the porous medium to a desired outlet or region of the device, by means of capillary action.
What is a microfluidic assay?
The term microfluidics refers to devices, systems, and methods for the manipulation of fluid flows with characteristic length scales in the micrometer range7. Microfluidic systems display fundamentally different properties from everyday perceptions of how fluids behave.
What is microfluidic paper-based analytical devices?
Microfluidic paper-based analytical devices (μPADs) are a new class of point-of-care diagnostic devices that are inexpensive, easy to use, and designed specifically for use in developing countries.
What is continuous flow PCR?
A micromachined chemical amplifier was successfully used to perform the polymerase chain reaction (PCR) in continuous flow at high speed. The device is analogous to an electronic amplifier and relies on the movement of sample through thermostated temperature zones on a glass microchip.
What is microfluidic extraction?
DNA extraction from a single cell. The microfluidic device is designed to capture individual cells in an array of micropillars, perform lysis, extraction, purification, and then partially linearize the released human chromosomal DNA.
What are microfluidic products?
A microfluidic chip is a set of micro-channels etched or molded into a material (glass, silicon or polymer such as PDMS, for PolyDimethylSiloxane). The micro-channels forming the microfluidic chip are connected together in order to achieve the desired features (mix, pump, sort, or control the biochemical environment).