What are microsatellites markers?
Microsatellite markers are co-dominant, polymorphic DNA loci containing repeated nucleotide sequences, typically with 2 to 10 nucleotides per repeated unit.
How do you develop Kasp markers?
Development and Analysis of KASP Markers Each KASP reaction was carried out using a 3-μL reaction mixture consisting of 1.48 μL KASP 2 × reaction mix, 50 ng DNA template, 0.17 μM Hex forward primer, 0.17 μM FAM forward primer and 0.42 μM universal reverse primer.
How do you analyze microsatellite?
Microsatellite marker analysis involves PCR amplification of the microsatellite loci using fluorescently labeled primers that flank the repeated sequence. The labeled PCR products are then analyzed by CE to separate the amplicons by size.
Why are microsatellites useful?
Microsatellites provide data suitable for phylogeographic studies that seek to explain the concordant biogeographic and genetic histories of the floras and faunas of large-scale regions. They are also useful for fine-scale phylogenies — up to the level of closely related species.
How many microsatellites are in the human genome?
600,000 unique microsatellites
Each microsatellite consist of a short motif (1–6 base pairs) repeated in tandem to form an array [2]; over 600,000 unique microsatellites exist in the human genome [3, 4].
What is allele specific PCR?
Allele-specific polymerase chain reaction (AS-PCR) is a technique based on allele-specific primers, which can be used to analyze single nucleotide polymorphism (SNP) effectively including the transition, transversion and insertion/deletion polymorphism and has been exploited in the study of diseases research, molecular …
What do microsatellites tell us?
How do microsatellites work?
Microsatellites are simple sequence tandem repeats (SSTRs). The repeat units are generally di-, tri- tetra- or pentanucleotides. For example, a common repeat motif in birds is ACn, where the two nucleotides A and C are repeated in bead-like fashion a variable number of times (n could range from 8 to 50).
What is a microsatellite marker in genetics?
Repetitive segments of DNA scattered throughout the genome in noncoding regions between genes or within genes (introns). They are often used as markers for linkage analysis because of their naturally occurring high variability in repeat number between individuals.
What are microsatellite DNA markers?
Microsatellite are powerful DNA markers for quantifying genetic variations within & between populations of a species, also called as STR, SSR, VNTR. Tandemly repeated DNA sequences with the repeat/size of 1 – 6 bases repeated several times
Can microsatellite markers be isolated from Cabomba aquaticas?
Microsatellite markers isolated from Cabomba aquaticas.l. (Cabombaceae) from an enriched genomic library. Appl Plant Sci. 2015;3:1500076–1500076.
Is there a microsatellite marker for Vitex rotundifolia?
Ohtsuki T, Shoda T, Kaneko Y, Setoguchi H. Development of microsatellite markers for Vitex rotundifolia(Verbenaceae), an endangered coastal plant in Lake Biwa, Japan. Appl Plant Sci. 2014;2:1300100–1300100.
What do microsatellite markers tell us about the Sweet Passion Fruit?
Microsatellite markers in sweet passion fruit, and identification of length and conformation polymorphisms within repeat sequences. Plant Breed. 2013;132:732–735.