What is NS2B and NS3?
The viral NS2B-NS3 serine protease (NS2B-NS3pro) is a promising target for the development of drug-like inhibitors, which are not available at the moment. In this study, we report retrocyclin-1 (RC-1) production in E. coli as a recombinant peptide to test against dengue NS2B-NS3pro.
What are the non structural proteins of dengue virus?
The 11kb RNA genome of the dengue virus encodes three structural proteins (envelope, pre-membrane, capsid) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5), all of which are translated as a single polyprotein that is subsequently cleaved by viral and host cellular proteases at specific …
What are the properties of dengue?
DENV infections may range from asymptomatic or mild to the more serious instances of dengue fever (DF), dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Symptoms include high fever, severe headache, generalized muscle and/or joint pain, rash, nausea, vomiting, etc.
What kind of enzyme is NS3?
The NS3 N-terminal domain is a chymotrypsin-like serine protease with an absolutely conserved catalytic triad His51, Asp75 and Ser135, while membrane bound NS2B serves as a cofactor essential for folding and catalysis12,13.
What does NS1 protein do?
NS1 is an important immunomodulatory factor whose primary role is to inhibit interferon production and counteract the activity of interferon-inducible proteins that limit influenza virus replication (Price et al., 2000).
What is the pathogenesis of dengue?
The pathogenesis of dengue was attributed to various viral and host factors such as non-structural protein 1 (NS1) viral antigen, DENV genome variation, subgenomic RNA, antibody-dependent enhancement (ADE), memory cross-reactive T cells, anti-DENV NS1 antibodies and autoimmunity.
Which pathogen causes dengue fever?
The pathogen Dengue is caused by a virus of the Flaviviridae family, Flavivirus genus which includes viruses such as yellow fever, West Nile and tick-borne encephalitis.
What cells does dengue virus infect?
The virus infects nearby skin cells called keratinocytes, the most common cell type in the skin. The dengue virus also infects and replicates inside a specialized immune cell located in the skin, a type of dendritic cell called a Langerhans cell.