What is an isoenzyme test?

The ALP isoenzyme test is a lab test that measures the amounts of different types of ALP in the blood. The ALP test is a related test. Blood is drawn from a vein (venipuncture), usually from the inside of the elbow or the back of the hand.

When is an ALP isoenzyme test necessary?

When the ALP test result is high, you may need to have the ALP isoenzyme test. This test will help determine what part of the body is causing higher ALP levels. This test may be used to diagnose or monitor: Bone disease.

What is the cause for the elevated isoenzyme?

Higher levels of the three isoenzymes mean different things: CK-MM generally rises if you have muscle damage in your heart, brain, or skeleton after a crush injury, seizures, muscular dystrophy, muscle inflammation, or another skeletal muscle disorder.

What is the role of isoenzymes?

Major function of isoenzymes is in the control of metabolic activities of the cell under different metabolic or environmental conditions which exist at different sites within the cell, in the same cell at different stages of its development or in different tissues and organs of the organisms.

What bone disorders cause high ALP?

High ALP levels can be caused by bone diseases, such as Paget’s disease, osteomalacia, rickets, bone tumors, or tumors that have spread from another part of the body to the bone, or by overactive parathyroid glands (hyperparathyroidism). Normal healing of a bone fracture can also raise ALP levels.

How are isozymes used in diagnosis of diseases?

Abstract. Serum enzymes and isoenzymes are of clinical interest because they can be used as molecular markers of tissue damage. Normally, cell membranes are impermeable to enzymes and hence enzyme activities in the serum are very low compared with those in cells.

What do isoenzymes do?

Isozymes (also known as isoenzymes) are enzymes that differ in amino acid sequence but catalyze the same chemical reaction. These enzymes usually display different kinetic parameters (i.e. different KM values), or different regulatory properties.

What is the diagnostic significance of isoenzymes?

Creatine kinase isoenzyme content is frequently used to assess the state of differentiation of muscle and neural tissue and following release into plasma as diagnostic markers for acute myocardial infarction, skeletal muscle disease, and neurologic injury.