What is Sepharose column?
The IgG binding capacity of Protein A Sepharose® Column is ≥ 16 mg human or rabbit IgG per mL of wet beads. Protein A Sepharose® Columns display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation.
How does a heparin column work?
Heparin column is sufficient to remove DNA from your protein because it serves as cation exchanger, charge repulsion with in the column will make DNA come down. If still you are worrying that protein is bound with DNA, I would recommend either (Polyethyleneimine)PEI precipitation.
How does heparin affinity chromatography work?
Heparin chromatography is an adsorption chromatography in which biomolecules can be specifically and reversibly adsorbed by heparins immobilized on an insoluble support. An advantage of this chromatography is that heparin-binding proteins can be conveniently enriched using its concentration effect.
How do you regenerate a heparin column?
Regenerate the column with 2-3 bed volumes of 8 M urea, 1.5 M NaCl in PBS. Follow the urea wash with 3-5 bed volumes of application buffer. The column is now ready for re-use. 7.
What is the composition of Sepharose?
For daily use, Sepharose High Performance media are stable in all common, aqueous buffers, 1 M NaOH, denaturing agents (8 M urea, 6 M guanidine hydrochloride), 70% ethanol, 1 M acetic acid, 30% acetonitrile and with additives such as non-ionic detergents.
What is heparin sepharose?
Heparin Sepharose® is prepared by covalently coupling heparin to epoxy-activated 6% cross-linked Sepharose® beads. The coupling was optimized to give a high binding capacity and could be greater than 0.4 mg of heparin-binding protein (such as thrombin) per ml of wet gel.
How do you clean a heparin column?
Cleaning. Remove ionically bound proteins by washing with 0.5 column volume 2 M NaCl for 10–15 minutes. Remove precipitated or denatured proteins by washing with 4 column volumes 0.1 M NaOH for 1–2 hours or 2 column volumes 6 M guanidine hydrochloride for 30–60 minutes or 2 column volumes 8 M urea for 30–60 minutes.
What is CM Sepharose?
CM Sepharose® Fast Flow is a weak cation exchanger based on the well established Sepharose® Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
What is Sepharose resin?
Sepharose™ is a spherical, agarose-based size exclusion chromatography resin. Sepharose is available with 2 different agarose contents, 4% and 6%, designated Sepharose 4B and Sepharose 6B respectively.
What column platforms are compatible with heparin Sepharose 6 fast flow?
Heparin Sepharose 6 Fast Flow is compatible with gravity columns, Tricorn columns, XK columns and HiScale columns. For process-scale applications, Cytiva recommends AxiChrom columns. Heparin Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow. Prepacked ReadyToProcess columns are available on request.
What is heparin Sepharose 6?
Heparin Sepharose 6 Fast Flow is an established BioProcess affinity resin for capture or intermediate purification of proteins with affinity for heparin. Heparin Sepharose 6 Fast Flow is an established BioProcess affinity resin for capture or intermediate purification of proteins with affinity for heparin.
How much Heparin is in a 5ml column?
• CONTENTS: Ready-to-use pre-packed columns of 1 ml or 5 ml bead volume in 20 % Ethanol/dH2O. > 2.5 mg heparin per ml Sepharose beads.
How do you make a heparin Sepharose solution?
Heparin-Sepharose is prepared by covalently coupling heparin to epoxy-activated 6% cross-linked sepharose beads. The coupling was optimized to give a high binding capacity and could be greater than 0.4 mg of heparin-binding protein (such as thrombin) per ml of wet gel. Why buy BioVision Products?