What is TCEP-HCl?

Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions.

Why is TCEP used?

TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis.

Is TCEP stronger than DTT?

For long-term storage of proteins, TCEP is significantly more stable than DTT without metal chelates such as EGTA in the buffer, whereas DTT is more stable if metal chelates are present.

Is TCEP a flame retardant?

Background on TCEP TCEP (CASRN 115-96-8) is a colorless liquid. The primary use for TCEP is as a flame retardant in paint and coating manufacturing.

What is the difference between DTT and TCEP?

For long-term storage of proteins, TCEP is significantly more stable than DTT without metal chelates such as EGTA in the buffer, whereas DTT is more stable if metal chelates are present. Thus TCEP has advantages over DTT, although the choice of reductant is application specific.

What is TCEP HCl used for?

What is the molecular weight of TCEP HCl?

Description TCEP•HCl, Tris(2-carboxyethyl)phosphine hydrochloride, 1g TCEP•HCl, Tris(2-carboxyethyl)phosphine hydrochloride, 10g Molecular Weight: 286.65 CAS #51805-45-9

What are the features of TCEP-HCl?

Features of TCEP-HCl: • Efficient—For most applications, 5 to 50 mM TCEP provides sufficient molar excess to effectively reduce peptide or protein disulfide bonds within a few minutes at room temperature.

What is the stability of TCEP solution?

Stability in Solution • TCEP is stable in aqueous, acidic, and basic solutions. When TCEP is dissolved directly in water, the resulting pH is approximately 2.5. Studies indicate that no change in concentration of TCEP occurs after 24 hour incubation at room