What is interaction proteomics?
The association of an unknown protein with partners belonging to a specific protein complex involved in a particular process would then be strongly suggestive of its biological function.
What are the three main activities of proteomics?
Proteomics relies on three basic technological cornerstones that include a method to fractionate complex protein or peptide mixtures, MS to acquire the data necessary to identify individual proteins, and bioinformatics to analyze and assemble the MS data.
What is network analysis in proteomics?
Network analysis, the evaluation of how nodes relate to one another, has over the years become an integral tool for analyzing high throughput proteomic data as they provide a structure that helps reduce the complexity of the underlying data.
What is proteomic testing?
Proteomics involves the applications of technologies for the identification and quantification of overall proteins present content of a cell, tissue or an organism.
What is the role of protein–protein interaction in functional genomics?
Protein–protein interactions govern cell physiology, and the disruption of some sensitive connections in the network can have pathological effects. Once a genome has been sequenced, one of the goals of functional genomics is the elucidation of the protein interaction network supporting biochemical and genetic pathways.
Does the peroxisomal membrane protein pex13p interact with SH3?
The peroxisomal membrane protein Pex13p shows a novel mode of SH3 interaction. EMBO J. 2000;19:6382–6391. [PMC free article][PubMed] [Google Scholar]
How do small domains affect protein-protein interactions?
A substantial proportion of protein interactions relies on small domains binding to short peptides in the partner proteins. Many of these interactions are relatively low affinity and transient, and they impact on signal transduction.
What are the blue dots in inferred protein interaction networks?
Inferred Protein Interaction Networks (A) Protein interaction network mediated by the SH3 domains of the proteins characterized in this study. The SH3-containing proteins are represented as blue dots, while the prey partner proteins are represented as black dots.