Why is it important that cultures are incubated at 25c and not 37c in a school laboratory?
Inoculated agar plates are incubated at 25°C in school laboratories for no more than 24–48 hours. This encourages growth of the culture without growing human pathogens which thrive at body temperature (37°C).
Why is it recommended to incubate the plates at 35 37 C?
At 35°C, most human bacterial pathogens with differing optimum growth temperature will grow reliably, although colonies may appear small or require additional incubation due to their slower growth rate. DST is recommended to be performed at 35°C ± 1°C.
Why are these plates incubated at 25 C rather than at 37 C?
Why are nutrient agar plates incubated at 37 degrees C and Sabouraud agar at 25 degrees C. Both optimum temps. 37 = human body temp = temp at which some of bodies bacteria will grow. 25 = optimum temp for fungi as low pH kills bacteria anyway.
Why is 37 degrees the optimal incubation temperature?
For example, although both pathogens and environmental bacteria found indoors are mesophiles, pathogens will grow faster than environmental strains at 37 degrees Celsius (98 degrees Fahrenheit), the temperature of the human body.
Why were you told to incubate the plates at 30 C when the human body temperature is 37?
Do not incubate at human body temperature (or above 30 °C) – this reduces the risk of culturing microbes that are pathogens to humans.
Why is the plate incubated at 37 C?
Selective Growth For example, although both pathogens and environmental bacteria found indoors are mesophiles, pathogens will grow faster than environmental strains at 37 degrees Celsius (98 degrees Fahrenheit), the temperature of the human body.
What happens to bacteria at 37 degrees?
Under the proper conditions (usually 37 degrees Celsius), the bacteria will consume the agar as food and grow into colonies called colony forming units (CFU’s). The Revolutionary Science Incufridge is a chilling incubator that can be used as a standard bacterial incubator for growing bacterial colonies on petri plates.