What is binding site of promoter?

A promoter is a sequence that initiates and regulates the transcription of a gene. Protein binding sites in a promoter represent the most crucial elements and the corresponding proteins are called transcription factors (TFs).

What is the binding site for regulatory protein?

Each operon contains regulatory DNA sequences, which act as binding sites for regulatory proteins that promote or inhibit transcription.

What is YY1 gene?

YY1 (YY1 Transcription Factor) is a Protein Coding gene. Diseases associated with YY1 include Gabriele-De Vries Syndrome and Insulinoma. Among its related pathways are mTOR signaling pathway and Sterol Regulatory Element-Binding Proteins (SREBP) signalling.

How do you identify transcription factor binding sites?

Prediction of transcription factor binding sites is generally performed by scanning a DNA sequence of interest with a position weight matrix (PWM) for a transcription factor of interest [6, 7] and various pattern-matching tools have been developed for this purpose.

What is motif in DNA sequence?

Sequence motifs are short, recurring patterns in DNA that are presumed to have a biological function. Often they indicate sequence-specific binding sites for proteins such as nucleases and transcription factors (TF).

How does a protein recognize a DNA sequence?

Proteins recognize a particular sequence by having a surface that is chemically complementary to that of the DNA, forming a series of favorable electrostatic and van der Waals interactions between the protein and the base pairs.

What are regulatory DNA sequences?

A regulatory sequence is a segment of a nucleic acid molecule which is capable of increasing or decreasing the expression of specific genes within an organism. Regulation of gene expression is an essential feature of all living organisms and viruses.

What is CTCF binding site?

CTCF-binding sites are located at both active and inactive domain boundaries,24 and some are also located at the borders of the lamina-associated domains, where transcriptional activity is low.

What is the difference between ATAC seq and ChIP seq?

ATAC-seq is a high-throughput sequencing method for the study of chromatin accessibility. ChIP-Seq combines the selectivity of ChIP with the power of next-generation sequencing (NGS), providing genome-wide profiling of DNA targets for DNA-associated proteins.