What is the purpose of KIA test?
Kliger’s Iron Agar (KIA) This is a differential medium. It tests for organisms’ abilities to ferment glucose and lactose to acid and acid plus gas end products. It also allows for identification of sulfur reducers.
What is the purpose of kligler iron agar test?
Kligler Iron Agar is used for the differentiation of microorganisms on the basis of dextrose and lactose fermentation and hydrogen sulfide production in a laboratory setting. Kligler Iron Agar is not intended for use in the diagnosis of disease or other conditions in humans.
Which pH indicator is used in kligler’s iron agar?
Phenol red
Kligler Iron Agar, in addition to peptone, HM peptone B and yeast extract, contains lactose and glucose (dextrose), which enables the differentiation of species of enteric bacilli. Phenol red is the pH indicator, which exhibits a colour change in response to acid produced during the fermentation of sugars.
What four results should be noted for the KIA test?
KIA Color Reactions: Red slant/ yellow butt – dextrose (+), lactose (-) Yellow slant/ yellow butt – dextrose (+), lactose (+)
What is the pH indicator in KIA?
Unlike TSI, sucrose is absent in KIA. Sodium thiosulfate is the source of the sulfur and ferrous sulfate acts as a hydrogen sulfide detector. Phenol red indicator is yellow below a pH of 6.8….Composition of Kligler’s Iron Agar.
| Ingredients | Gram/Liter |
|---|---|
| Phenol red | 0.024 gm |
| Distilled water to equal 1 L | |
| Final pH : 7.4 |
How do you inoculate a KIA?
With a straight inoculating needle, inoculate KIA by stabbing through the center of the medium to the bottom of the tube and then streaking the slant while withdrawing the needle. Incubate tubes aerobically with loose caps at 35-37ºC for 18-24 hours and examine the reaction of the medium.
What is methyl red test?
The Methyl Red test involves adding the pH indicator methyl red to an inoculated tube of MR-VP broth. If the organism uses the mixed acid fermentation pathway and produces stable acidic end-products, the acids will overcome the buffers in the medium and produce an acidic environment in the medium.
Does E coli test positive methyl red?
Escherichia coli: MR test positive- the appearance of red color after the addition of methyl red reagent. Klebsiella (formerly Enterobacter) aerogenes: MR test negative- the lack of color change after the addition of methyl red.