What is slide agglutination?
Slide Agglutination. The slide agglutination technique is used to determine which of several bacteria agglutinates a preparation of selected antibodies. Emulsions of unknown bacteria are mixed with drops of known antibodies on a micro- scope slide, and the mixture is observed for agglutination.
What is widal slide agglutination test?
Widal Test (Slide Agglutination) test helps to detect typhoid fever and paratyphoid fever – collectively known as enteric fever, caused by the consumption of food or water contaminated by Salmonella bacteria.
What is slide agglutination test in microbiology?
A rapid slide agglutination test has been developed for the identification of Neisseria gonorrhoeae that are primarily detected as oxidase-positive colonies in gonococcal cultures. The technique is based on the specific nonimmune reactivity between the Fc portion of immunoglobulin (Ig)G and staphylococcal protein A.
What was our slide agglutination test positive for?
Of 10,000 sera examined for infectious diseases by both the rapid slide agglutination and the tube agglutination tests, 128 were found positive for typhus fever and 257 for typhoid and paratyphoid infections.
What is principle of slide agglutination test?
In principle, the test is performed in a similar manner to other well known slide tests. A certain amount of concentrated killed antigen and patients serum are mixed on a plate, slide or card and allowed to react for a specified period, after which the presence of agglutination is determined by naked eye.
What is agglutination?
Agglutination is defined as the formation of clumps of cells or inert particles by specific antibodies to surface antigenic components (direct agglutination) or to antigenic components adsorbed or chemically coupled to red cells or inert particles (passive hemagglutination and passive agglutination, respectively).
How is typhoid confirmed?
Testing for typhoid fever A diagnosis of typhoid fever can usually be confirmed by analysing samples of blood, poo, or pee. These will be examined under a microscope for the Salmonella typhi bacteria that cause the condition. The bacteria aren’t always detected the first time, so you may need to have a series of tests.
What is O and H in Widal test range?
The procedure of the Widal test:
| The titer of the antibody | Interpretations of Widal test |
|---|---|
| O-antigen = >1:160 | Active infection |
| H-antigen = >1:160 | Past infection or immunized |
| Rising titer = 1:40 to 1:80 to 1:160 | Diagnostic |
What is the purpose of agglutination?
Agglutination is commonly used as a method of identifying specific bacterial antigens and the identity of such bacteria, and therefore is an important technique in diagnosis.
How do you read agglutination test?
The results are read directly from the slide. The subject is blood group A if agglutination occurred with the Anti-A test serum; group B if agglutination occurred with the Anti-B test serum; group AB if agglutination occurred with both test serums, and O if there was no agglutination in either case.
What is agglutination used for?
Agglutination tests are frequently used for initial confirmation of specific pathogens. Since antibodies to the target organism may cross-react with other organisms and autoagglutination may occur, these must be considered as screening tests and further confirmation will usually be necessary.