How is flow cytometry used in cancer research?
A laboratory method that measures the number of cells, the percentage of live cells, and certain characteristics of cells, such as size and shape, in a sample of blood, bone marrow, or other tissue. The presence of tumor markers, such as antigens, on the surface of the cells are also measured.
What are the clinical applications of flow cytometry?
Flow cytometry is most commonly indicated for both benign and malignant hematologic processes. It can aid in several clinical areas, including diagnosis, treatment plans, and monitoring residual or relapsed disease ((Craig & Foon, 2008; (Wood et al., 2007).
What is the most common clinical application of flow cytometry?
The most common application performed on the cytometer is immunophenotyping. This technique identifies and quantifies populations of cells in a heterogeneous sample – usually blood, bone marrow or lymph.
What is the purpose of a flow cytometry analysis?
Flow cytometry provides a well-established method to identify cells in solution and is most commonly used for evaluating peripheral blood, bone marrow, and other body fluids. Flow cytometry studies are used to identify and quantify immune cells and characterize hematological malignancies. They can measure: cell size.
What cancers can flow cytometry detect?
Flow cytometry is a laser-based technique used to detect and analyze the chemical and physical characteristics of cells or particles….Results and Follow-Up
- Multiple myeloma.
- Non-Hodgkin lymphomas (both B-cell and T-cell).
- Acute myeloid leukemia.
- Acute lymphoblastic leukemia (ALL).
- Chronic lymphocytic leukemia (CLL).
Why would a doctor order a flow cytometry?
Flow cytometry can provide information that is used to diagnose, stage and monitor blood cancers. It can also be used to test for minimal residual disease (MRD), the number of cancer cells remaining in the body after treatment.
What are the advantages of flow cytometry?
The two greatest advantages of flow cytometry are its ability to measure a large number of parameters (2 to 30 or more) on the same sample and its ability to collect information from millions of cells in a matter of seconds.
What is flow cytometry and how does it work?
Flow cytometry is a technology that rapidly analyzes single cells or particles as they flow past single or multiple lasers while suspended in a buffered salt-based solution. Each particle is analyzed for visible light scatter and one or multiple fluorescence parameters.
How accurate is flow cytometry?
The diagnostic accuracy of FC was 88.4%, sensitivity was 85.8%, and specificity was 92.9%. In addition, FC accuracy for classes of non-Hodgkin lymphoma was assessed. We conclude that FC is an independently accurate ancillary test in the evaluation of FNA.
Why would a doctor order a flow cytometry test?
When is a flow cytometry test necessary? Flow cytometry may be used to characterize and count types of white blood cells in the evaluation of infectious diseases, autoimmune disorders or immunodeficiencies. It’s also used to diagnose and classify leukemia or lymphoma.
What does flow cytometry diagnose?
Flow cytometry. Flow cytometry is often used to test the cells from bone marrow, lymph nodes, and blood samples. It’s very accurate in finding out the exact type of leukemia or lymphoma a person has. It also helps tell lymphomas from non-cancer diseases in the lymph nodes.
What do the results of my flow cytometry mean?
– Fever – Bleeding that doesn’t stop or that soaks through the bandage – Pain that gets worse with time – Swelling at the site of the biopsy – Redness or drainage at the biopsy site that gets worse with time.
How do you analyze flow cytometry results?
Purpose of Test. Flow cytometry is used in many areas of clinical testing.
– The main advantage is the ability to characterize antigen expression (i.e. immunophenotyping) on a cell-by-cell basis on large populations of cells. – Fairly quick to run (on the order of several hours). – Relatively inexpensive. – Well understood.