What is the function of DNA polymerase 1?
DNA polymerase I functions to fill DNA gaps that arise during DNA replication, repair, and recombination. DNA polymerase II also functions in editing and proofreading mainly in the lagging strand (Kim et al.
What is the function of DNA polymerase 1 quizlet?
What is the function of DNA polymerase I? Proofreads DNA. (about 20 at a time before it dissociates). It is a 5′ to 3′ DNA polymerase but also has 5′ to 3′ and 3′ to 5′ exonuclease activity to “proof read” and “correct” mismatches.
What are the subunits of DNA polymerase?
Abstract. The DNA polymerase III holoenzyme is composed of 10 subunits. The core of the polymerase contains the catalytic polymerase subunit, alpha, the proofreading 3′–>5′ exonuclease, epsilon, and a subunit of unknown function, theta.
What is the function of DNA polymerase 1 and 2?
DNA polymerase I and II have a role to play in repair, removing the primer and filling the gaps. In eukaryotes, DNA polymerase 𝝳 is the main enzyme for replication. Other DNA polymerases are involved in the repair, proofreading and primer removal.
What would happen without DNA polymerase 1?
DNA polymerase I is strikingly important for survival of the cell following many types of DNA damage, and in its absence, the cell has persistent single-stranded breaks that promote DNA recombination.
What is the role of DNA ligase in the elongation of the lagging strand during DNA replication?
What is the role of DNA ligase in the elongation of the lagging strand during DNA replication? It joins Okazaki fragments together.
What are the three functions of DNA polymerase?
DNA pol I — replaces the RNA primer with DNA. DNA pol II — DNA proofreading. DNA pol III — replicates the majority of the DNA on both the leading and lagging strands.
How does polymerase 1 differ from polymerase 3?
The main difference between DNA polymerase 1 and 3 is that DNA polymerase 1 is involved in the removal of primers from the fragments and replacing the gap by relevant nucleotides whereas DNA polymerase 3 is mainly involved in the synthesis of the leading and lagging strands.